FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118791
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGGTGTATAAAACACT45950.41071120522063553No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGAGGTCCCT38420.34340640923997423No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31920.285307979774596No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25960.23203618906480297No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25910.23158927806891547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22990.205489675909084No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22970.205310911510729No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22720.20307635653129139No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20910.18689817848016296No Hit
ACATTATAGTAGTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20850.18636188528509792No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20760.1855574454925004No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20420.18251845072046521No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20280.18126709993198015No Hit
GTTTTATACACCTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA20180.18037327794020508No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19920.17804934076158999No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19810.1770661365706374No Hit
TCCTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCACTGAATCGGTCAGGGAC19770.17670860777392738No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18780.1678597700553544No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18750.1675916234578219No Hit
GTCTCTGGTGGGTCCCTCGATGGTTACTACTGGAACTGGATCCGCCAGCC18400.1644632464866092No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18240.16303313129976912No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17700.15820649254418387No Hit
GTAATAAACCGCCACATCCTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCA17490.15632946636145623No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17090.15275417839435607No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCAC16880.15087715221162842No Hit
GTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC16860.15069838781327344No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16840.15051962341491842No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16550.14792753963877078No Hit
GGCTGAGGATGTGGCGGTTTATTACTGTCACCAACATTATAGTAGTCCTT16490.14739124644370577No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.14560360246015563No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16250.14524607366344564No Hit
GTTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCACTGAATCGGT15860.14176016789552293No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15800.14122387470045789No Hit
GTCTTGGACCTCCTGCACAAGAACATGAAACACCTGTGTTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15500.13854240872513277No Hit
CTATAATGTTGGTGACAGTAATAAACCGCCACATCCTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTT15450.13809549772924523No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAATCTCAGGGAGA15020.13425206316461252No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14930.13344762337201496No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14890.13309009457530496No Hit
GTCAGGGACCTCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA14630.1307661573966898No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14580.13031924640080228No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14270.12754839822629965No Hit
ACTATAATGTTGGTGACAGTAATAAACCGCCACATCCTCAGCCTGCAGGC14260.12745901602712215No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14220.12710148723041212No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA14160.12656519403534708No Hit
CACCATCAACAGCCTGCAGGCTGAGGATGTGGCGGTTTATTACTGTCACCAACATTATAGTAGTCCTTGGACGTT13960.12477755005179697No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGGTG13950.12468816785261949No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA13940.12459878565344198No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13790.1232580526657794No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT13500.12066596888963176No Hit
ATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAACAGCC13370.11950400030032418No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13320.11905708930443668No Hit
CATTATAGTAGTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13260.11852079610937163No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13250.11843141391019413No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAATCTCAGGGA13220.11816326731266162No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12700.11351539295543137No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12570.11235342436612379No Hit
GTTTATTACTGTCACCAACATTATAGTAGTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12440.11119145577681623No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12300.10994010498833115No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12220.10922504739491112No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12050.10770555000889352No Hit
TTATAGTAGTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT11980.107079874614651No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA11970.1069904924154735No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA11920.10654358141958596No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11910.10645419922040845No Hit
ATAGTAGTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA11900.10636481702123095No Hit
GTGTATTATTGTGCGAGGCACTATTACGATTTTTGGAGTGGTTATTACGG11780.10529223063110088No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11740.10493470183439088No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11490.10270014685495325No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11390.10180632486317821No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11360.10153817826564569No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11280.10082312067222564No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11210.1001974452779831No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11200.1001080630788056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32700.041.3969541
GTGGTAT5100.040.990071
GGTATCA13050.039.1286431
TATCAAC36400.037.174062
TGGTATC5550.037.0341642
ATCAACG39350.034.56133
TTAACGG501.8588467E-434.5079935
TATTAGA501.9404534E-434.2566032
AACGCAG40350.033.874566
CAACGCA40350.033.7047655
TCAACGC40250.033.703394
AGAGTAC42550.032.04260611
CGCAGAG43050.031.8295968
CAGAGTA43200.031.56048210
GCAGAGT43800.031.2845699
GTCTATA450.00477611830.4625681
ACGCAGA46050.029.8303977
TAACGGC605.393376E-428.77609336
TATTCTA1155.771243E-428.44219470
AGTACGG39700.028.38897713
Produced by FastQC (version 0.11.5)