FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696006
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC126470.74569311665171No Hit
GTATAGGAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT121160.7143842651500054No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63090.37199160852025287No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACTCTCACCATCAGCAGC47490.28001080184857835No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47250.27859571251516796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42890.25288825629154615No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41390.24404394795773127No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC40570.2392090594019125No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40050.23614303251285668No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39460.2326642712348895No Hit
GGGTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCA38820.22889069967912848No Hit
CTCTAAACTAGACGCCTCATACATGATGAGGGTAGGGGGTTTCCCTGGTT37930.22364307673439832No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG36740.21662659212290525No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36570.2156242371784062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33970.20029410273312712No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32960.19433893512169179No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA32550.1919214908437824No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32340.1906832876770483No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31480.18561255089899445No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30000.17688616667629714No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA29760.17547107734288678No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29720.1752352291206517No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28440.16768808600912968No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT28190.16621403462016054No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27170.16019990495316644No Hit
CCCCCTACCCTCATCATGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAAT27010.15925651206422617No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26720.15754661245302198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24450.14416222584118218No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAGGAGTTAC24440.1441032637856234No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23960.14127308511880263No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23840.14056554045209746No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23760.14009384400762734No Hit
TGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACT23240.1370278171185715No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22940.13525895545180855No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22670.13366697995172186No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22660.1336080178961631No Hit
CAGTATAGGAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22450.13236981472942902No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21860.12889105345146185No Hit
CCTCATACATGATGAGGGTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCAATAGTCTGA21820.12865520522922677No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21190.12494059572902454No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21090.12435097517343688No Hit
GACTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAACCCCCTACCCTCATCATGTATGAGGCGT20340.11992882100652945No Hit
CCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC20240.11933920045094179No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCCTCCACC20170.11892646606203043No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19340.11403261545065288No Hit
GAGTGAATTCTGTCCCAGATTCACTGCCGCTGAACCTTGATGGGACCCCA19210.11326610872838894No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19130.11279441228391882No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGTAACT18990.11196894350609608No Hit
ATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAACCCCCTAC18490.1090208407281578No Hit
CATCATGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATT18110.1067802826169247No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17780.10483453478348545No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17730.10453972450569161No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17290.1019453940611059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8000.040.2983441
GTATCAA45550.038.927051
TATCAAC51400.034.683512
AGGGACC29450.032.60197431
AACGCAG54700.032.5910876
CAACGCA55450.032.086555
TCAACGC55850.031.7321154
ATCAACG56250.031.6283443
ACTTTCG30150.031.40781220
CGGAGGG30750.031.4010428
GAGGGAC31350.031.25974530
AGAGTAC56250.030.53590811
ATCAGGC33350.030.53172336
TTTCGGC31100.030.34345822
GGACCAA31850.030.28114133
CAGAGTA57050.030.16780710
TTCTGTC37700.030.11160569
TACCCGC31650.030.0055611
TACGGAT2651.2732926E-1129.88928670
TATAGGA33150.029.7846012
Produced by FastQC (version 0.11.5)