FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643630
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19950.039.8177641
TTAGACC350.001369071939.3006483
CTAGCAT451.02041384E-438.208974
TATCAAC22200.035.9370772
GGTATCA9150.035.7034571
CGGTCTA400.00258828334.51954334
CTTATAC1253.6379788E-1233.0125471
AACGCAG26150.031.2977436
CAACGCA26150.030.9032345
TCAACGC26600.030.3804364
ATCAACG26500.030.3653133
AGAGTAC26050.029.94950511
CAGAGTA26150.029.83497610
GGACTAG703.934687E-529.4754871
GCAGAGT27550.029.1921319
ACGCAGA28150.028.9451967
GTGGTAT3450.028.905911
CGCAGAG28650.028.1956588
TGGTATC3500.027.5104562
GACTAGG756.29719E-527.5104542
Produced by FastQC (version 0.11.5)