FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643630
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11320.1758774451159828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23400.040.8365971
GTGGTAT3150.040.374931
GTATAGA451.02300706E-438.19251
CCGTTAA451.02394384E-438.18656516
TATCAAC25900.036.7535552
GGTATCA9850.035.245671
TGGTATC3650.034.8362
TCGTAAG400.002645780334.3652422
AACGCAG29200.032.5999036
CAACGCA29550.032.330075
TTTAACG652.3042336E-531.87779234
AGAGTAC28950.031.81307411
CAGAGTA29050.031.70356210
ATCAACG30150.031.6842253
ATAGCAC553.332536E-431.23873
ACGCAGA30500.031.2103967
CGATTAC450.004403640530.97037347
GCAGAGT30550.030.934349
TCAACGC30950.030.865254
ATTAACC450.00471051630.5468795
Produced by FastQC (version 0.11.5)