FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269506
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG250.001366391779.1482570
CTTTAAT208.56447E-568.9185516
AAAATTG300.00281624865.9568770
ACTTTAA309.192805E-657.42985515
CTTATAC1100.056.38791
TAATTCG252.582311E-455.13483419
TTATACA1400.049.2294732
TATACAC1300.047.7165953
TTAATTC306.348551E-445.94569818
GGTATCA12000.044.222731
TATAGCA405.0361377E-543.0757872
CTCGTAT1450.042.99719639
GTATCAA31750.042.3279231
TACACAT1600.040.923615
TTTAATT350.001355720239.38202717
TATCAAC35700.037.8391232
ATACACA1650.037.5948944
GTGGTAT3500.036.4283751
TGGTATC3600.033.5033872
AGAGTAC41450.032.1705411
Produced by FastQC (version 0.11.5)