FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269506
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14170.11161822000053563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAA150.002368198768.90726522
CTTTAAT200.007415751551.64991416
ACTTTAA200.007415751551.64991415
TGGTATC5950.043.3998682
GTGGTAT6250.041.3264431
GTATCAA35550.039.621461
GGTATCA14600.038.4486851
TATCAAC39400.035.3044322
AGACTAT501.876732E-434.4522721
TTATTCC400.002622214134.4305654
ATAAGAG1056.566552E-1032.791013
TATAAGA1056.566552E-1032.791012
TAAGAGA1151.7407729E-929.9396214
AGAGTAC45450.029.85092411
CAGAGTA45950.029.52610410
AACGCAG47550.029.4728576
GCAGAGT48200.028.9336629
GTATAAG1202.737579E-928.6989171
CAACGCA49200.028.4122125
ACCCTAA500.00780713427.54770313
Produced by FastQC (version 0.11.5)