FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77244
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCAA200.007249991451.8992235
GACTAGC200.0074199151.59630626
AGACTAG200.007438970751.56286625
GAGACTA200.007438970751.56286624
TGGGGTT200.007458068451.5294721
TATACAC200.007458068451.529473
TGTACAG200.007458068451.529475
CCGTTTG200.007458068451.5294715
CCTTGTG200.007458068451.529479
GGTATCA1150.044.8082351
GTCCAGA400.002636053134.352981
GTATCAA2050.033.5151021
TATCAAC2100.032.717122
GGCAGGA450.00440296430.93671448
ACATCTC450.004693335430.535988
AGAGTAC2450.028.04324711
CAGAGTA2450.028.04324710
CACGCCT500.00785327.4823829
AACGCAG2600.026.4253676
CAACGCA2800.024.537845
Produced by FastQC (version 0.11.5)