FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291156
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25650.1986591860317421No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21460.16620764648113784No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20050.1552871999975216No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18300.14173345436182772No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17830.13809330553395563No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17390.13468550663126685No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16370.12678560917503384No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14710.11392891331488991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.041.015521
GTATCAA53450.040.858091
TATCAAC61900.035.442312
GTGGTAT6300.035.316521
TTATACA2150.031.8380322
TATACAC2150.031.8380323
AACGCAG68650.031.8078846
ATCAACG69850.031.4574343
CAACGCA69850.031.4084345
TCAACGC70700.031.1760524
CTTATAC2100.030.9698721
ACTAGAC450.00480684330.4230083
AGAGTAC71850.029.6279411
CAGAGTA72050.029.49705310
GCAGAGT74900.029.105749
CGCAGAG75900.028.7222638
ACGCAGA81550.026.775317
TGGTATC8600.026.2663762
AGTACGG64750.024.52824813
GAGTACG65500.024.29776412
Produced by FastQC (version 0.11.5)