FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129514
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27750.039.8899771
GGTATCA12000.039.6569751
TGTATAA952.2191671E-1036.29125214
TATCAAC32400.034.1574632
TAGGGTC1207.8216544E-1131.6036344
TGGTATC3750.031.2588652
TTATACA1359.094947E-1230.6459482
TATACAC1359.094947E-1230.6459483
AGAGTAC34400.030.46507511
CAGAGTA34500.030.27684810
AACGCAG36900.029.9919176
GTGTATA1151.715307E-929.9797313
GCAGAGT36700.029.7769579
CAACGCA37300.029.7627225
ACTAAGG1401.4551915E-1129.56715827
CTTATAC1401.4551915E-1129.5579911
ATCAACG38100.028.8663083
GTGGTAT4550.028.8000971
CGCAGAG38400.028.3701888
ACGCAGA39550.027.8067687
Produced by FastQC (version 0.11.5)