FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129514
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16920.14979894007511194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5950.040.5386772
GTGGTAT6350.039.0791
GTATCAA31950.038.942251
TATCAAC35150.035.389172
GGTATCA13100.034.9916951
CGATAGT501.7579534E-434.83726552
AACGCAG39300.031.5644686
CAGAGTA38350.031.44926310
AGAGTAC38250.031.4413911
CAACGCA40900.030.4981695
GCAGAGT40900.030.2467619
ATCAACG41550.029.7709523
TCAACGC42450.029.5456124
ACGCAGA43200.028.8757067
CGCAGAG42850.028.7898858
TCATATG500.007737500627.59806828
CAGGTAT1457.1122486E-1026.1462481
CTCCTAG1457.1122486E-1026.1462481
AGTACGG46450.024.62975713
GAGTACG47500.024.15785612
Produced by FastQC (version 0.11.5)