FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115409
Sequences flagged as poor quality0
Sequence length20-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.11063206411280527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2350.051.0360371
TATACAC2450.050.349333
TTATACA2600.047.4424362
AGTAATG405.0347353E-543.0772233
TAATCTC2350.042.58299635
ATACACA3000.041.1186184
AGTAATC2450.040.79149233
TAGTAAT2950.039.67750532
TTTAGGG350.001393747739.160593
TGTATAG350.001394055139.15883621
GTATCAA35100.038.3673821
GTAGTAA3250.037.0425231
TACACAT3350.036.8226435
TCGTATG3400.035.70563540
AGGGGGG3300.035.5261970
GTAATCT2850.035.08852434
GGTATCA14600.034.736461
CTCGTAT3300.034.63830639
TATCAAC39350.034.3945922
GTGTATA2250.031.9825821
Produced by FastQC (version 0.11.5)