FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414472
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGG65020.45967682640589563No Hit
GTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGAAA60680.4289939991742502No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC49810.35214553557794004No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47950.3389957524786634No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT45320.3204022419673207No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44100.31177711541833275No Hit
GTCTCTGGTGGCTCCGTCACCAGTAATAGTTACTACTGGGGCTGGATCCG40030.2830031276688404No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39840.2816598702554734No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG33630.2377565621659531No Hit
GTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGGGCCCCATAGCTGGTTCGACCCCTGGGG31750.22446538354947992No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29630.20947745872664852No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC29550.20891187665786243No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA28790.20353884700439456No Hit
CAGTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGA28610.20226628734962585No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27750.1961862801101754No Hit
CACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGACCT27700.1958327913171841No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27350.19335836976624493No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC27170.19208581011147624No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG26610.1881267356299736No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGA25680.18155184408033528No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA25590.1809155642529509No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC25540.18056207545995961No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT25480.18013788890837004No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG24480.17306811304854391No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24410.17257322873835607No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA24400.17250253097975782No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23750.16790717667087082No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23360.16514996408553864No Hit
GTCTTCAGGCTGCAGGCTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACCTCCACTGAACCTTGATGGGAC22330.15786809494991771No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22280.1575146061569264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22200.15694902408814032No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT22200.15694902408814032No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA22130.15645413977795247No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA21990.15546437115757683No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG21760.15383832270981682No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG21630.1529192518480394No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC21130.14938436391812634No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20820.14719273340158023No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20070.14189040150671064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19970.14118342392072802No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG19710.1393452821971732No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG19530.13807272254240452No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC19320.13658806961184103No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT19160.13545690547426883No Hit
CACTTGCACTGTCTCTGGTGGCTCCGTCACCAGTAATAGTTACTACTGGG18860.133335972716321No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC18720.13234620409594533No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18710.13227550633734708No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC18550.13114434219977492No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT18280.12923550271762183No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC18250.12902340944182705No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA18150.12831643185584443No Hit
CCGTAGATCAGGACCTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAAGT18140.12824573409724618No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18020.12739736099406707No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG17860.12626619685649487No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC17840.12612480133929832No Hit
GGTCAGGACATTACCAACTCTTTAACTTGGTATCAGCACAAACCAGGGAA17810.12591270806350358No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC17790.12577131254630705No Hit
GTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACCTCCA17550.12407456633994876No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17470.12350898427116266No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17370.12280200668518006No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG17280.12216572685779571No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT17260.12202433134059917No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC17170.12138805151321481No Hit
ACCAGAGACAGTGCAAGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGATGCAG16720.11820665237629306No Hit
CCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAGCTGACCTCT16620.11749967479031044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16600.11735827927311393No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG16590.11728758151451565No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAG16490.11658060392853306No Hit
CAGTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACCTCCA16270.1150252532393713No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC16090.11375269358460259No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGCTATGGGGCCCAAATAACCACTCCAAGTCTCGGGCGACAGACCCCG15950.11276292496422693No Hit
CATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACA15840.11198524961964607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15780.11156106306805648No Hit
GGTCAGGCTGACCTGGTTCTTGGTCAGCTCATCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTG15460.10929873479291212No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15260.10788477962094689No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGCACACGTCCGAGAACCAGTTTTCCCTGAAG15210.1075312908279556No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACT15190.10738989531075907No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTC15150.10710710427636602No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA15040.10632942893178514No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGCACACGTCCGAGAA14980.10590524238019558No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCAGGCGGGTCAGGACATTACCAA14960.10576384686299906No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG14900.1053396603114095No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT14720.10406710065664078No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC14720.10406710065664078No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14660.10364291410505122No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC14410.10187547014009468No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14350.10145128358850511No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCA14350.10145128358850511No Hit
GATCTACGGTGCATCCAATTTAGAAACAGGGGTCCCATCAAGGTTCAGTGGAGGTGGATCTGGGACAGATTTTA14310.10116849255411206No Hit
ATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC14280.10095639927831727No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14260.10081500376112075No Hit
GATATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGCTCCCACT14240.10067360824392424No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC14170.1001787239337364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)