FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428746
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33850.2369210482479041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTAG207.849206E-570.1434956
GTATCAA42500.035.6187971
TGTTAAG400.00266473334.3180962
TATCAAC46500.032.325432
TAATCGT901.9318213E-730.78075638
CACTAAG450.004725516330.52954924
GATTATC703.5175704E-529.96717852
GGTATCA22950.029.7645971
ACCTTTA952.7751412E-729.53410556
GTGGTAT9200.029.4760231
AACGCAG51600.029.3995956
TTAATAG1103.0189767E-828.79600160
AGAGTAC50500.028.61274511
CAGAGTA51050.028.3044810
TCCTATA2700.027.9628932
GCAGAGT53400.027.9586899
TTAATCG1004.876838E-727.69094737
CAACGCA55850.027.0385325
CGCAGAG56250.026.7251648
ACGCAGA56950.026.5172067
Produced by FastQC (version 0.11.5)