FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468627
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA7490.15982860569280047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6880.14681185676454836No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6870.1465984674378557No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6070.1295273213024431No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5750.12269886284827806No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC4740.10114654085231964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC200.00747268651.5449875
GTGGTAT1850.048.299561
GGTATCA6450.044.2241941
GTATCAA17800.042.476061
TATCAAC20600.036.694782
GTATAGG501.9024333E-434.3669931
TGGTATC2500.034.359662
ATCAACG22800.033.308313
CAACGCA22900.033.0128025
TCAACGC23250.032.663634
AACGCAG23150.032.5078546
AATAGCG901.6291597E-731.37570462
AGAGTAC23650.031.22938711
CGCAGAG24500.030.569828
ACGCAGA24850.030.2807467
CAGAGTA24400.030.26946610
AGATAAC605.5411924E-428.6391621
TAATCGT854.3153323E-628.49997938
TTAATCG854.3515774E-628.46937637
ATACCGC500.00788561227.4877267
Produced by FastQC (version 0.11.5)