FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087136
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57810.27698242951106206No Hit
CTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT47920.22959692133143217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41490.1987891541327446No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC40390.1935187740520982No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39480.18915873234901798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38600.18494242828450086No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37070.1776118087177836No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35960.1722935160909495No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35440.1698020636891894No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33650.16122571792159207No Hit
ACCTACTACAACGCGTCCCTCAAGAGTCGAGTCACCATATCTGTAGACACGTCCAAGAACCAGTTCTCCCTGCAA33610.16103406773684129No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC33580.16089033009827822No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTG32900.157632276957515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32760.15696150131088726No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG31580.1513078208607393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31450.15068495776029928No Hit
GGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT30370.14551040277202826No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG29950.1434980758321451No Hit
CTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT29600.1418211367155758No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28620.1371257071891817No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTT27720.13281357803228921No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA26590.12739946031307972No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26470.12682450975882742No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT26400.12648912193551354No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25740.1233268938871257No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25670.12299150606381185No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25640.12284776842524875No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAA25590.12260820569431029No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25340.12141039203961793No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25260.12102709167011635No Hit
GTAGTAGGTGGTCCCATCATAATAGATTCTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC25070.12011675329255016No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24800.11882311454548243No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA24680.11824816399123009No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT23500.11259448354108213No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23100.11067798169357435No Hit
GGCCAAAAGTGTACAAGGGGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTG22560.10809070419943885No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22480.10770740382993729No Hit
GTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT22440.10751575364518651No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22370.10718036582187265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21710.10401813777348483No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21440.10272449902641706No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT21420.10262867393404168No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21380.1024370237492909No Hit
GAAATACACAGCCGAATCAGCGGCGGTCACAGAGCTCAGTTGCAGGGAGA21240.10176624810266316No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20900.10013722153228155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)