FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742887
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11220.151032391198123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7500.040.3835531
GTATCAA17850.038.177731
GTGGTAT2700.036.9672661
TGGTATC2750.036.292692
TATCAAC21300.031.8302822
TAAACGT450.00438752230.99422850
AACGCAG22000.030.971856
CAACGCA21900.030.7969265
CAGAGTA22050.030.12330210
AGAGTAC22050.029.96722211
TCAACGC22600.029.6907794
ATCAACG22900.029.3018153
CGCAGAG24700.028.1454548
GCAGAGT24150.027.78899
GGCTTAT1005.119309E-727.5342391
ACGCAGA24850.027.4215937
TAGAGTG851.4681781E-424.2900125
AGTACGG25200.023.35336313
GAGTACG25650.022.94365512
TACGGGG10350.022.27712215
Produced by FastQC (version 0.11.5)