FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014193
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17780.1753117996278815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG700.001917294336.69558370
GTATCAA22350.035.680411
GCGATTA601.9386111E-434.26095669
GGTATCA10850.034.214751
GTGGTAT4450.033.986941
TATCAAC26550.029.899212
AACGCAG28050.028.4242346
ATCAACG28350.028.1192913
CAACGCA28950.027.6551975
TCAACGC28900.027.5841484
CGCCTTA950.00854628127.0388570
AGAGTAC29500.026.91061611
TGGTATC6150.026.8212132
CAGAGTA30050.026.64680110
ACGCAGA30300.026.5403657
CGCAGAG32250.024.7224738
GCAGAGT32850.024.4801549
CTACACT1455.5007877E-721.3276984
TACGGAG1305.0912695E-621.1474932
TAGAGTG1004.4537507E-420.6167745
Produced by FastQC (version 0.11.5)