FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341012
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30010.22378621518673955No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15440.11513692644062841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6650.036.0746881
GTATCAA31200.034.9300081
GGTATCA15400.034.716031
TGGTATC7300.030.9731452
TATCAAC36300.030.0112932
TCAACGC37250.029.2448144
ATCAACG37750.028.9482123
CAACGCA37900.028.9251085
AACGCAG38300.028.5335716
AGAGTAC39800.027.19995911
ACGCAGA40400.027.1351877
CAGAGTA41450.026.36515610
TATACCG659.0208225E-426.3524426
CGCAGAG41900.026.0819998
GCAGAGT42550.025.6835679
GTATAAC1001.6466867E-523.9896681
AGTACGG37250.023.91174713
TCTAGAC1301.9784602E-723.7163143
GAGTACG38750.022.9861312
GTAGTTT3506.475602E-1021.8587870
Produced by FastQC (version 0.11.5)