FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201113
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18050.15027728448530653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34300.039.101531
GTGGTAT6250.035.7648621
TATCAAC38400.034.826922
GGTATCA14000.034.6349331
AACGCAG43150.030.838926
CAACGCA43400.030.6587265
ATCAACG43850.030.4199733
TGGTATC7250.029.4002062
TCAACGC45050.029.3807324
ACGCAGA45450.029.2807527
CAGAGTA44200.029.25180210
AGAGTAC44300.029.10815211
GCAGAGT46550.028.2933439
CGCAGAG47600.027.596988
CTACACT951.0797799E-525.3320964
AGTACGG48750.023.6296513
GTACCTA1052.338267E-522.9261951
GAGTACG50650.022.67535612
TACGGGC14250.021.9581415
GTACGGG52850.020.75375214
Produced by FastQC (version 0.11.5)