FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725413
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9230.1272378631207326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGA150.002234510469.9212151
CTCATAT150.002362579368.945536
TAGGCTA200.00662541153.1405760
CTTAGAT200.006945106752.51115852
GTCTTAA200.007583573551.356151
CTAAACA200.00759391651.338454
CTGCTAA405.2307467E-542.7967951
TTAACAA350.001361058139.3482635
GTCTATA451.0472656E-438.0415951
GTATCAA23000.036.3214531
TGGTATC4400.035.7813452
GTGGTAT4550.034.613671
TATTCAC400.00257135134.5663440
GGTATCA10650.034.3981741
GCTCTAG400.00269478534.2374341
GTAGGGT501.9501182E-434.2256363
TATCAAC24950.033.1968082
GTATCAG553.4050093E-431.124941
TGTGTGA553.4077637E-431.12064710
GCTAAAC450.0048056430.4227873
Produced by FastQC (version 0.11.5)