FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128167
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTTA302.7445565E-586.6970370
TTTTAAG250.001445916678.02733670
CTTATAC1050.055.7291451
TTATACA1200.054.4974022
GGTATCA13050.045.6308251
TATACAC1400.044.2535323
GTATCAA35200.042.83061
ATACACA1550.039.9709364
GACTTAG350.001338029939.48733
TATCAAC38450.039.4771422
AGGATTA350.001363390239.336475
TACACAT1700.036.4440845
GTAACAA400.00262568734.4209441
AGAGTAC45300.032.74347711
CAGAGTA45450.032.55969210
AACGCAG46900.032.2911346
GCAGAGT48450.031.2539319
CAACGCA48500.031.1548925
ATCAACG49600.030.7415353
ACTTAGG450.00456834230.74083534
Produced by FastQC (version 0.11.5)