FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128167
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21780.19305652443299617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTAG150.002317055169.2871738
CTTTTAT200.006890495352.6180560
TAAGACT200.007121073552.18190845
ACAATAT200.00728393651.8843535
ACTGTAA356.6749455E-445.47951569
GTATCAA39150.042.81731
GGTATCA14350.041.9766921
GTGGTAT4650.040.7128831
TATCAAC41900.039.9178662
TGAGTAT350.001360728539.35211623
GAGTATA400.002618864234.4392124
TATGTTC501.8842201E-434.4285219
GTGTTAG501.8866763E-434.4208871
TGGTATC5200.034.4147872
TGTATAA807.250128E-834.4147832
CAGAGTA48450.033.9560910
AACGCAG50400.033.736446
AGAGTAC49000.033.57495511
CAACGCA51100.032.936055
GCAGAGT50600.032.922879
Produced by FastQC (version 0.11.5)