FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463720
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67290.45971907195365236No Hit
GTACAATAACTGGCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT56930.38894050774738337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45580.3113983548766157No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCAGTA43450.29684639138633073No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42170.2881015494766759No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42110.28769163501216083No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38630.26391659607028667No Hit
GTTATTGTACTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT38420.2624818954444839No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC36770.2512092476703195No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36700.2507310141283852No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT36650.25038941874128934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36590.24997950427677423No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT36230.24752001748968383No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35490.2424644057606646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33950.2319432678381111No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32930.22497472194135493No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30930.2113109064575192No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27990.1912250976962807No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27870.19040526876725056No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27390.18712595305113No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGCAGCAGCTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCT26760.18282185117372174No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAG25780.17612658158664224No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCAGTACAATAACTGG25240.1724373514060066No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24890.17004618369633537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23780.16246276610280655No Hit
CTCCCAGGCTCCTCATCTATGGTTCTTCCACCAGGGCCACTGGTATCCCA23580.16109638455442296No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23140.15809034514797912No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23140.15809034514797912No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22380.15289809526412157No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21410.14627114475446124No Hit
GTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAGCAGCTTAGCCTGGTA21190.14476812505123932No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20670.141215533025442No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20040.13691143114803378No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19550.13356379635449403No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19480.13308556281255976No Hit
GGATACCAGTGGCCCTGGTGGAAGAACCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGG19350.13219741480611047No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18840.12871314185773236No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18690.12768835569644468No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18640.12734676030934877No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA17970.12276938212226382No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17780.12147131965129943No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17590.12017325718033504No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16900.11545924083841172No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16690.11402454021260897No Hit
GACTATTAGCAGCAGCTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTTCTTC16680.1139562211351898No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15830.1081490995545596No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCAGTACAATAACTGGCCTCAGACGTT15700.10726095154811029No Hit
GCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTTCTTCCACCAGGGCCACTGGTATCCCAGCCAGGTT15480.10575793184488837No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATCGTGATGACGCAGTC15410.10527969830295411No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15410.10527969830295411No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC15300.10452818845134315No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15180.10370835952231301No Hit
GCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGCTAAGCTGCTGCTA15160.10357172136747465No Hit
GCTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATC14990.10241029705134863No Hit
CATCTATGGTTCTTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14970.10227365889651026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)