FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503538
Sequences flagged as poor quality0
Sequence length20-76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTAC150.002369093568.8945921
CACTAGT150.002370960868.8809051
GTATACG252.5877828E-455.1047251
TGTGATT200.00740124451.67094419
ACTAGTA200.0074099751.6555522
GTATACC200.0074099751.6555524
TATACCG200.0074099751.6555525
GTATCAA15300.043.89471
TATACGA405.0521692E-543.046292
TATACAC405.0521692E-543.046293
GTGGTAT2100.042.640561
TATCAAC16950.039.617832
GGTATCA5250.036.7364851
TGGTATC2600.035.7615362
AGAGTAC18750.034.70908411
CAGAGTA18850.034.70762310
AACGCAG20200.033.755116
GCAGAGT20250.033.328349
ATCAACG21350.031.7756233
CAACGCA21500.031.3937595
Produced by FastQC (version 0.11.5)