FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503538
Sequences flagged as poor quality0
Sequence length20-76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9860.19581441718400597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGA150.0023756868.846382
CTTGTAT208.603986E-568.8463752
GTGTATA252.5916568E-455.088041
TGTATAA252.5942014E-455.07712
CTAACTT200.00739266151.68610822
GTCTTAG200.00741593551.6450351
TTGCTAT400.00831774650.16035570
GTGGTAT2650.046.7728651
TGGTATC3100.039.9753152
GTATCAA15600.038.402721
TATAAGA655.5389864E-737.0711252
TATCAAC16800.035.6525882
GGTATCA6550.035.2185021
GTATAAG709.877385E-734.4300271
TCCGGTA400.002623473334.4231872
CTTTACC450.003479207932.49586568
ATAAGAG751.6960275E-632.1283073
AGGAGTA652.3545515E-531.7752512
AGTACAG553.2972632E-431.2938045
AGAGTAC18650.030.63940611
Produced by FastQC (version 0.11.5)