FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038196
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16390.15786999757271267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAG150.002383778668.7925221
CGTTTAT200.006815309652.7639261
ATATATC200.00743138351.62174225
GTATAAG1450.049.800871
TATAAGA1500.048.1338842
TAAGAGA1550.046.5789384
ATAAGAG1600.045.123343
GGTATCA13350.045.075691
GTATCAA35300.041.5947761
TATCAAC38450.038.0922052
AGAGTAC42500.033.5739911
CAGAGTA42600.033.41446710
AACGCAG44100.033.133956
GCAGAGT44150.033.0201459
CAACGCA45100.032.473945
GTGGTAT4300.031.1875861
ATCAACG47200.030.9562873
CGCAGAG47850.030.6105738
ACGCAGA48750.030.1865147
TCAACGC49650.029.4287384
Produced by FastQC (version 0.11.5)