FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293347
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38740.29953291730680165No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT29920.23133776163705486No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29810.23048725516044805No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25280.19546185207836722No Hit
CTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG24460.18912171288911636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24150.18672483100049714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23530.18193106722325872No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC22830.17651875328121533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22610.17481774032800168No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21830.16878687622115335No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20970.16213746194950002No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20750.16043644899628637No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT20400.1577302920252647No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA20070.15517877259544421No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG18290.14141603142853387No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA17890.13832328060450907No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17860.13809132429270723No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17810.1377047304397041No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACT17680.13669958642189606No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCC17340.13407074822147497No Hit
CCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTG17190.13291096666246566No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17140.13252437280946258No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16340.12633887116141299No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16020.12386467050219314No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15940.12324612033738817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15780.12200902000777827No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15750.12177706369597639No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCC15340.11860699410135098No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14610.11296272384750575No Hit
GTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT14300.11056584195888652No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCT14220.10994729179408155No Hit
CTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA14090.10894214777627349No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14050.10863287269387101No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT14030.10847823515266977No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13840.107009178511258No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13770.10646794711705367No Hit
CTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTC13590.1050762092462425No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13530.10461229662263878No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13270.10260200858702266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)