FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293347
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36590.2829093816276684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32890.254301436505439No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26490.20481742332104222No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT26400.20412155438563664No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23510.18177642968205748No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC23500.18169911091145688No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23170.1791475914816364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22910.17713730344602027No Hit
CTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG22800.17628679696941346No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22120.1710291205685713No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22060.1705652079449676No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA20110.15548804767784669No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19360.1496891398828002No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18900.1461324764351717No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18680.14443146348195804No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18200.1407201624931283No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACT17970.13894183076931405No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG17930.13863255568691155No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA17650.1364676301100942No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17470.13507589223928304No Hit
GTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT16380.12664814624381546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15890.1228595264843851No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15480.11968945688975968No Hit
CCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTG15400.11907090672495471No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCC15350.11868431287195161No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14970.11574619958912805No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14930.11543692450672557No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCT14770.11419982417711565No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14390.11126171089429208No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT14060.10871019146447164No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCC13970.10801432252906606No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13950.10785968498786482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13680.10577207818164808No Hit
CTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTC13510.10445765908143755No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13450.10399374645783381No Hit
CTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA13230.10229273350462018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.040.6179241
GTATCAA24850.038.615631
GTGGTAT3950.035.572821
TGGTATC4150.034.680262
TATCAAC28650.033.1311342
ATCAACG30200.031.202553
AACGCAG30550.030.9620256
CAACGCA30650.030.8574285
TCAACGC30600.030.7946744
CGTTTCG500.006480504328.61891466
AGAGTAC33100.028.1625811
CAGAGTA33150.028.120110
CGCAGAG33900.028.0034528
ACGCAGA35000.027.0264727
TGTTAGA7450.025.02898870
TATAAGA2400.024.2727412
AGTACGG31950.023.9202413
GAGTACG32950.023.40230412
GCCGTTA902.223909E-422.84846715
TACTAGA902.2262394E-422.8449332
Produced by FastQC (version 0.11.5)