FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225495
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGG150.002383779568.774851
TGACAAC150.002385877568.7595915
GTATCAA6550.043.57491
GTGGTAT856.730261E-1140.455791
TATCAAC7100.040.1904642
AGTACCG350.001368800339.29125
GTATAAG451.0184199E-438.2082441
TATAAGA451.01975165E-438.199772
TACACCG754.1209205E-836.671785
TGGTATC952.2555469E-1036.1892552
CAACGCA8350.034.5856635
GGTATCA2200.034.3874241
AGGAGTA400.002636671334.3797952
AGAGTAC8100.034.37979511
TACTGGA501.8951605E-434.3797952
TAAGAGA501.8951605E-434.3797954
CAGAGTA8150.033.7470410
ATCAACG8500.033.5708583
AACGCAG8550.033.374546
TACGGGT1253.6379788E-1233.00460415
Produced by FastQC (version 0.11.5)