FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225495
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3210.14235348899088673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1050.045.8760721
GTATCAA6550.040.4479871
GGACAGA350.001364965739.313637
GGTATCA2600.038.3770981
TATCAAC7000.037.8393672
TGGTATC1350.035.6734732
GTCCTGT400.002626443334.4070551
CCTACAC501.8888153E-434.3994263
AACGCAG7950.034.1830756
AGAGTAC7550.034.1716111
CAGAGTA7550.034.1716110
ACGCAGA8250.032.523097
GCAGAGT8250.032.523099
CAACGCA8550.031.7842625
ATCAACG8750.031.0577663
CGCAGAG9050.030.0282278
TCAACGC9100.029.8632354
TACGGGT1650.029.1873915
CAAGTCC500.007401494327.84061249
ACGGGTG1208.697134E-825.79956616
Produced by FastQC (version 0.11.5)