FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433101
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7740.17871120131331952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTTT300.00289948565.4599470
TACTATC200.007499226451.4981272
CGTTACA306.460547E-445.7761152
GTATCAA12500.042.8662341
GTGGTAT1650.041.6338731
GGTATCA4850.039.659481
TGGTATC1750.039.2366682
TATCAAC14000.038.255752
CAAATAC400.002398656435.05652655
CTCCTAG701.0059139E-634.3479421
AGAGTCG807.37964E-834.3360511
AACGCAG16450.033.1880346
AGAGTAC15950.032.50622611
CAACGCA16900.032.503755
GCAGAGT16800.032.4966169
CAGAGTA16000.032.40464410
TAGCATA450.003848066331.83306767
ATCAACG17250.031.247173
AAATACC450.004254339331.1870956
ACGCAGA17700.031.0382357
Produced by FastQC (version 0.11.5)