FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190066
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2050.043.7141761
GTCTTAG405.0305054E-543.083681
GTATCAA43550.042.025141
TATACAC2200.040.730243
GGTATCA16350.039.2101061
TATCAAC47700.038.510142
TTATACA2450.036.5740932
AACGCAG53500.034.2707946
ATCAACG54900.033.647963
CAACGCA54750.033.55135
CAGAGTA53150.033.19263510
AGAGTAC53000.033.1565511
TCAACGC56150.033.0216564
GCAGAGT55900.032.6692479
ACGCAGA56300.032.560917
TACACAT2450.032.3540045
CGCAGAG57550.031.733938
GTGGTAT6350.030.9388351
ACCTAAC450.004646391630.634713
TGGTATC6700.029.320162
Produced by FastQC (version 0.11.5)