FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190066
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20690.17385590379020996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46150.043.819121
TATCAAC51750.039.0009352
GGTATCA16450.036.4401861
GTGGTAT7300.036.338341
TATCGTA400.002476976534.8327449
GTCCTAG807.1844624E-834.4506381
GTATATA400.00261466534.4506381
AACGCAG59400.034.3288736
TGGTATC7700.033.5473562
CAACGCA60550.033.4465185
ATCAACG60850.033.2816243
AGAGTAC58850.033.1864511
CAGAGTA59250.032.9624110
ACGCAGA62500.032.6812677
TCAACGC62300.032.4503674
GCAGAGT62000.032.3892679
CGCAGAG63750.031.7703068
TAAGACT802.799161E-630.135444
TCTAACA703.8956292E-529.5216732
CAACTAG500.00770970427.61854731
Produced by FastQC (version 0.11.5)