FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362867
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.042.224121
GTATCAA38500.041.7378651
TATCAAC45250.035.3596882
TGGTATC6000.032.68872
CAACGCA50550.032.130015
AACGCAG50700.032.102826
TATACAC1950.031.7634663
ATCAACG51300.031.5261153
TCAACGC52550.030.7762094
AGAGTAC51750.030.37975711
CAGAGTA52350.030.0972810
CTTATAC1950.029.9977281
ACGCAGA54450.029.7622137
CGCAGAG54500.029.5433448
GTGGTAT6450.029.3411431
TAGACTA605.505843E-428.6753525
GCAGAGT58700.027.6043289
TTATACA2550.024.2888182
AGTACGG58600.023.07306513
GTACACT750.002023569322.940284
Produced by FastQC (version 0.11.5)