FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873635
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT208.574801E-568.8993453
TATAGCA150.00236903168.899342
ATCCTTA306.206933E-446.15487736
GGTATCA12000.040.7677761
CTTATAC350.00135696439.3733061
GTATCAA27950.039.197221
TATCAAC30400.036.4894522
GTGGTAT3150.036.0921971
TATGACC400.002614645334.4496734
TTATACA400.002614645334.4496732
TATACAC400.002614645334.4496733
GTATAAG1253.6379788E-1233.0735781
TGGTATC3600.032.53582
TGTGTAT553.292114E-431.30533412
CTCGTAT450.00451606330.8124739
CTAAGAC450.004621409330.66758530
CCCTAAG450.00463958830.64298428
TAGCAAG450.00463958830.64298428
CAACGCA36450.030.243885
AACGCAG36700.030.037866
Produced by FastQC (version 0.11.5)