FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873635
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13080.14971927635683094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTA150.00236803968.9066223
GTTTAGA200.007396274751.68292224
ATTGTAA200.007396274751.68292224
AATATAC200.007428162751.6267174
GGTATCA10300.044.4576841
TGTAGGA651.160879E-842.3652342
GTATCAA27900.042.0806661
GTATAAG1150.041.914341
ATAAGAG1150.041.899953
GTGGTAT3150.041.5341641
TATAAGA1250.041.3061032
TGGTATC3200.039.800152
TATCAAC30950.038.0363162
TAAGAGA1300.037.0653344
GCCCTTA400.002331706235.26347465
TTTAGAC400.002609622434.46317725
AGAGTAC33250.034.16685511
CAGAGTA33600.033.91340310
GCAGAGT35800.032.598639
AACGCAG36300.032.244456
Produced by FastQC (version 0.11.5)