FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456838
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18910.12980166634862628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA208.3459934E-569.27978538
ATTTGTG350.00497478957.13216470
CATATAG200.007462945851.567495
CTAGGTA250.00985334348.06586569
TATAAGA1000.041.2525752
GTATCAA43100.040.28641
GTATAAG1050.039.294911
TGCTAAA451.0229614E-438.1968272
GGTATCA17850.037.9465561
GTGGTAT7900.036.5591851
TATCAAC48050.036.058442
TTTGTTA400.002630839134.4078524
GTATAAC400.00264015934.3830451
TATAGGA701.00141E-634.3771482
TGGTATC8000.033.5177152
AACGCAG56800.030.9921056
ACACTCC450.004629131430.65847831
TATAGAG450.00470052530.5627067
GCAGAGT56600.030.5559549
CAGAGTA54300.030.52049310
Produced by FastQC (version 0.11.5)