FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283215
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19480.15180620550726107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43100.040.9399451
GTGGTAT7050.039.5187381
TATCTAG350.001369351339.3019875
TTAACCG350.001369351339.3019875
TATCAAC47300.037.371582
GGTATCA17050.035.908961
TATGCAA400.0024750134.83858549
TGGTATC8000.034.3905752
TTAAGGC400.002638216634.3878974
AACGCAG54500.032.5618866
CAGAGTA53250.032.55756810
AGAGTAC53300.032.3979511
GCAGAGT55550.031.6417799
TAGACTC450.004184487331.29750662
TGTATAA1101.0950316E-931.2641642
CAACGCA57450.030.7677425
ATCAACG57400.030.6735253
TGTAAGA450.00469528530.5694032
ACGCAGA58050.030.4556627
CGCAGAG59150.029.8311358
Produced by FastQC (version 0.11.5)