FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834125
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1150.047.7815633
TTATACA1100.046.8313562
TCGTATG1050.046.2453240
CTTATAC1200.042.9287451
GTGGTAT3400.040.4035221
TGGTATC3600.040.0668262
AGGGGGG1607.6397555E-1140.06346570
GGTATCA10550.040.03971
GTATCAA25200.039.52171
ATACACA1450.035.5272374
CTCGTAT1300.034.65461739
TATCAAC29100.034.2249762
TTACCAT750.002713031834.1874970
TACACAT1600.032.1965565
GGGTTAG652.3942595E-531.7012251
TACATAA553.1719654E-431.50419839
AACGCAG32150.030.8713076
CAACGCA32750.030.3057255
AGAGTAC31900.030.0348611
CAGAGTA32250.029.70890410
Produced by FastQC (version 0.11.5)