FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834125
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17890.21447624756481343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26500.041.0703771
GGTATCA10950.040.1338841
TTACGCC451.0317576E-438.1389434
TATCAAC30600.035.3367272
TTAGACC400.002661454534.325054
AACGCAG33250.032.6217046
TAACCCG500.003614383332.2461269
CAACGCA34050.031.855265
CAGAGTA33650.031.82971810
AGAGTAC33850.031.64165311
TAGCGTA450.004226786631.2319558
GCAGAGT35000.030.7981689
ACGCAGA35600.030.4719567
ATCAACG35600.030.4683063
TCAACGC35750.030.1484384
ATAAGAG1600.030.034423
CGCAGAG36350.029.5599148
GTATAAG1551.8189894E-1228.7956581
GTTCTAA605.5767834E-428.611071
CTAATTA500.00724949227.9680352
Produced by FastQC (version 0.11.5)