FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292335
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGGTCCTGTCTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43200.33427865065946527No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42350.32770140869047115No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33700.2607682992412958No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32330.25016733277362296No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGGAGGCTGCTGATGGCGAGAGTGAACT31800.2460662289576619No Hit
GTCTGGGACAGAGTTCACTCTCGCCATCAGCAGCCTCCAGTCTGAAGATT31270.24196512514170088No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGACACCAGTGGCCCTGGT31240.241732987189854No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27170.21023960505596456No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27020.20907891529673034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26270.20327546650055908No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25470.19708512111797638No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25120.19437684501309643No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24400.18880553416877202No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24050.1860972580638921No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24020.18586512011204526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23360.18075808517141453No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23180.17936525746033344No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22140.17131780846297592No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21740.1682226357716846No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGAC21370.1653596010322401No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCACCAATTTAGCCTGGTACCAGCAGAAACCTGGGCAGTCTCCCAGGCT21010.16257394561007787No Hit
CTCCCAGGCTCCTCATCTATGATTCATCCACCAGGGCCACTGGTGTCCCA20070.15530028978554322No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAC19410.1501932548449125No Hit
GAGTTCACTCTCGCCATCAGCAGCCTCCAGTCTGAAGATTTTGCAGTTTA18100.14005656428093335No Hit
GTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCACCAATTTAGCCTGGTA16990.13146746006259985No Hit
CCAGTGGCCCTGGTGGATGAATCATAGATGAGGAGCCTGGGAGACTGCCC16770.12976511508238964No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16110.12465808014175891No Hit
GCCTGGGAGACTGCCCAGGTTTCTGCTGGTACCAGGCTAAATTGGTGCTA15970.12357476969980695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15890.12295573516154867No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15700.12148552813318528No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT15540.12024745905666874No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15280.11823559680732938No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14610.11305118254941637No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14550.11258690664572267No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14450.11181311347289982No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14170.10964649258899588No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14050.10871794078160849No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13870.10732511307052738No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13860.1072477337532451No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13600.10523587150390573No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG13480.10430731969651831No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13460.10415256106195375No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13390.10361090584097776No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13230.10237283676446123No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12950.10020621588055728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)