FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292335
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61280.474180456305834No Hit
GTATAATGACTGGGTCCTGTCTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43460.33629051290880463No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41680.3225169944325581No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33810.2616194717314009No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGGAGGCTGCTGATGGCGAGAGTGAACT32880.2544231952241485No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGACACCAGTGGCCCTGGT30180.23353077955793194No Hit
GTCTGGGACAGAGTTCACTCTCGCCATCAGCAGCCTCCAGTCTGAAGATT30080.2327569863851091No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26280.20335284581784133No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25850.20002553517470315No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25700.19886484541546892No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25340.19607918999330667No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24790.19182332754278109No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24670.1908947757353937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24260.18772222372682004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23510.18191877493064879No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22570.17464511910611413No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGAC22420.17348442934687985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22250.17216898095308103No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20440.15816332452498771No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCACCAATTTAGCCTGGTACCAGCAGAAACCTGGGCAGTCTCCCAGGCT20130.1557645656892369No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20070.15530028978554322No Hit
GAGTTCACTCTCGCCATCAGCAGCCTCCAGTCTGAAGATTTTGCAGTTTA19640.151972979142405No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19100.1477944960091617No Hit
CTCCCAGGCTCCTCATCTATGATTCATCCACCAGGGCCACTGGTGTCCCA19000.14702070283633886No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18840.14578263375982234No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAC18440.142687461068531No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17790.13765780544518255No Hit
GTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCACCAATTTAGCCTGGTA17150.1327055291391164No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16790.12991987371695418No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15770.12202718335416127No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15380.1190093899801522No Hit
CCAGTGGCCCTGGTGGATGAATCATAGATGAGGAGCCTGGGAGACTGCCC15360.11885463134558764No Hit
GCCTGGGAGACTGCCCAGGTTTCTGCTGGTACCAGGCTAAATTGGTGCTA14680.11359283777039235No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14450.11181311347289982No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13850.10717035443596282No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13760.10647394058042225No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13390.10361090584097776No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13330.10314662993728407No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13330.10314662993728407No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13230.10237283676446123No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG13140.10167642290892066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13130.10159904359163839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG407.9218193E-443.92547269
CGATTGT1004.9118444E-639.943470
GTATCAA31900.037.1312451
GGTATCA14700.033.286931
TATCAAC36250.032.57612
ATCAACG37000.031.7309443
TCAACGC37300.031.4757354
CAACGCA37600.031.3143255
AACGCAG39050.030.3278736
CGCAGAG41700.028.5623878
AGAGTAC41750.028.44736511
GAGTACG29200.028.0788512
GCAGAGT42500.027.8659639
AGTACGG29600.027.69940413
TTATACG756.580213E-527.33645235
TGAGTCG500.00851689427.06073226
ACGTGTC3350.026.17822563
TGTTAGA13450.025.73800570
GTACGGG32050.025.47670214
CGAACTA1201.0121221E-725.41874129
Produced by FastQC (version 0.11.5)