FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899590
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28920.32147978523549614No Hit
GTCCCCACACCTCCTGATCTTTTTGGGTTCTAATCGGGCCTCCGGGGTCC26260.29191075934592425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21140.23499594259607154No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17920.19920185862448447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17840.19831256461276806No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17250.19175402127635924No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16760.18630709545459598No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15880.17652486132571504No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14940.16607565668804677No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14450.16062873086628354No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14190.15773852532820506No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14000.1556264520503785No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13860.15407018752987472No Hit
AGCCAGGGCAGTCCCCACACCTCCTGATCTTTTTGGGTTCTAATCGGGCC13800.1534032170210874No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13640.1516246289976545No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG13420.14917907046543424No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA13340.1482897764537178No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12780.14206471837170265No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12540.1393968363365533No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12350.13728476305872675No Hit
GTGTGGGGACTGCCCTGGCTTCTGCAGGTACCAATTAAAATAGTTGTATC12320.13695127780433308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12260.13628430729554575No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG12040.13383874876332552No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11570.12861414644449137No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11430.1270578819239876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11020.12250025011394079No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT10510.11683100078924843No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA10500.11671983903778387No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10260.11405195700263454No Hit
CTACAATCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCG10060.11182872197334341No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9730.10816038417501306No Hit
GGCCAAAAGTGTACGGAGATTGTAGAGCTTGCATGCAGTAATAAACCCCA9640.10715992841183206No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA9490.10549250213986372No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9480.10538134038839916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9390.10438088462521816No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9340.10382507586789537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9130.10149067908713968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCC200.003410953362.8446569
TAGTTAG703.3332093E-546.18717270
GTATCAA16750.040.363911
TATCAAC18300.037.11822
GGTATCA6300.034.8643261
CCTATAC400.002670193334.30243
AACGCAG19900.034.1338236
CAACGCA19800.034.1291545
TCAACGC19950.034.0444874
ATCAACG19850.034.043193
TGTTAGA4100.033.11958370
TAGATTG751.7452294E-632.0155755
AGAGTAC20950.031.4405411
GACAATT450.004639928730.6426134
CGCAGAG21700.030.5119788
CAGAGTA21700.030.51197810
ACGCAGA22750.029.4053277
GGGCTAT953.3528886E-728.900691
TATTAGT754.6185283E-528.79265267
GTGGTAT3350.028.6850131
Produced by FastQC (version 0.11.5)