FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058672
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGAT200.007281161451.8891236
ATAACCC200.007457878451.5753673
TTATACA800.047.277422
ATCGTTA306.420216E-445.84044621
ATACACA950.043.431894
TCTTATA405.10252E-542.9794732
GTATCAA32800.042.669021
TATACAC903.6379788E-1242.0243763
GGTATCA12350.040.6516341
CTTATAC955.456968E-1239.8163261
TATATGT350.001370351139.2955172
GTGGTAT3600.039.1627771
TATCAAC36200.038.6577842
GTATAGA451.02107704E-438.207591
TACACAT1100.037.509365
TGGTATC4150.033.969322
TATAAGA2250.033.61952
CAGAGTA40950.032.9919310
AGAGTAC41000.032.9516911
TAAGAGA2200.032.820694
Produced by FastQC (version 0.11.5)