FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058672
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19240.18173711971224327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACT150.002390487468.7438664
TTCTATA200.00746647851.5603372
CCTTAGT200.00746647851.5603372
ATAGGAT200.007467872551.5579033
AACACTA200.007467872551.5579035
GTAATTT305.9027947E-446.6286753
GTATCAA32600.039.332971
GCAATTA350.001367542539.3119222
ATACAAA451.0137599E-438.25427626
GGTATCA13550.037.54811
GTGGTAT4750.036.9098361
TATCAAC35400.036.3155672
TGGTATC5450.032.1660842
AACGCAG41550.030.9403386
GATAGAA850.004561010330.7482470
CAGAGTA40450.030.6784710
AGAGTAC40350.030.66930611
CAACGCA42000.030.6073915
TATGAGA450.004706297530.5542772
GCAGAGT43450.029.5082579
Produced by FastQC (version 0.11.5)