FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996055
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC10000.10039606246643007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23700.039.449791
TCGTATG1950.037.30042640
GGTATCA9600.036.163821
GTGGTAT4450.033.9873121
CTCGTAT2100.032.9634339
TCTCGTA2200.031.43965538
AACGCAG30000.031.3913426
ATCAACG30200.031.069643
CAACGCA30450.030.927435
CTTATAC2450.030.8660261
TATCAAC30950.030.7609462
TGGTATC5050.030.6268042
CGTATGC2400.030.33882941
TCAACGC31250.030.1356854
CAGAGTA31300.029.31741510
AGAGTAC31350.029.16102811
ACGCAGA32300.029.0481787
CGCAGAG33250.028.2182278
GCAGAGT33850.027.6165229
CGTAAAT657.8321894E-426.99689357
Produced by FastQC (version 0.11.5)