FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996055
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18880.18954776593661996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26200.038.9539151
GTGGTAT5050.036.7450071
TGGTATC5150.034.6830752
GGTATCA11050.033.8969151
CAACGCA32750.031.252365
ATCAACG32500.031.1757223
AACGCAG32800.030.9952936
TATCAAC33000.030.8105352
TCAACGC33150.030.7701054
ACGCAGA33700.030.0656057
CGCAGAG34500.029.1693278
AGAGTAC33500.029.1173211
CAGAGTA33500.029.1173210
GCAGAGT35600.027.9785929
TACGGGT5050.023.80305715
AGTACGG35900.023.63090513
CGTCTAA750.001947585623.09100740
GAGTACG36950.023.05234312
CTATCGG800.002966674521.4771161
TTTAACG2101.0004442E-1021.37240434
Produced by FastQC (version 0.11.5)