FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525259
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53210.3488587839835726No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37670.24697444827403084No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25810.1692171624622441No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT23870.15649801115744932No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22260.14594242682718148No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22170.14535236310685595No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21960.1439755477594297No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20570.13486234141217981No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20370.13355108870034532No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20060.13151864699700183No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19580.12837164048859898No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18400.12063524948877535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16960.11119422996356684No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT16780.11001410252291577No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16710.10955516407377369No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16490.10811278609075574No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT16370.10732603446365503No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16040.10516246748912808No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGGT15850.1039167774128853No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA15280.10017970718415692No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15260.10004858191297347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.042.0037881
GTATCAA47000.040.3148651
CACGGAT2700.038.06208870
TATCAAC50150.037.502942
ATCAACG52300.035.894793
TCAACGC53000.035.4207084
CAACGCA53600.035.0253565
AACGCAG55250.034.0422486
ACGACCG901.615148E-632.33651769
CGCAGAG59450.031.2363438
AGAGTAC61200.030.12003311
CGACTAA604.165841E-430.07456466
ACGCAGA63400.029.7209727
GCAGAGT62950.029.4453859
AATGGTA11600.029.18350870
GAGTACG37850.029.13062312
AGTACGG38550.028.69021213
CAGAGTA64600.028.64045510
CGACCGG1305.7233112E-527.90071570
TACGGGG20600.026.3494515
Produced by FastQC (version 0.11.5)