FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827559
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.041.008051
GTATCAA24250.038.4360121
GTGGTAT3600.033.4384651
TGGTATC3900.032.6261182
TATCAAC30600.030.2314622
ATCAACG30900.030.0492463
AACGCAG31050.029.904086
TCAACGC31900.028.9994584
CAACGCA32050.028.9710355
ACGCAGA33500.027.8180397
CAGAGTA33150.027.38395710
CGCAGAG34100.027.3285718
AGAGTAC33050.027.25873211
GCAGAGT34950.026.7607049
CGTTACT700.00114442525.29782365
TATACAC1452.0827429E-823.7170283
TCGTATG902.0465437E-423.12911440
TTAACGG1704.329195E-922.36975335
TATTAGA2351.8189894E-1221.9508672
GGCTTAC953.1291245E-421.7224311
Produced by FastQC (version 0.11.5)