FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827559
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11620.14041294940904517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT200.00703176252.3474550
TATACTG350.001379266839.24275
GTATCAA26950.036.9559941
GTGGTAT5300.035.6395721
GGTATCA11000.035.280231
TGGTATC5450.034.018232
AACGCAG32200.030.711686
TATCAAC32650.030.3898872
ATCAACG33350.029.7520183
TCAACGC33250.029.738244
CAACGCA33550.029.5782345
CAGAGTA33800.028.75339510
ACGCAGA34500.028.6676987
AGAGTAC33750.028.5924911
CGCAGAG34600.028.5848438
GACTCGT900.006979217828.18380770
GCAGAGT35350.028.075529
GTGTATA1353.2196112E-1027.9836641
TGTATAA1602.3264874E-923.6040842
TATTAGA2350.023.3758032
Produced by FastQC (version 0.11.5)